Homemade Colloidal Coomassie Blue Staining

Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250 Electrophoresis 1988 Jun 9(6):255-62 M Induced Uninduced Purified

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Coomassie brilliant blue G

CAS: 6104-58-1 MDL: MFCD00078482 Synonyms: Brilliant Blue G Acid Blue 90 Brillant blue G Brilliant indocyanine G Coomassie Blue G 250 Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]-2'-methylbenzhydrylene]-3-methylcyclohexa-2 5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium monosodium salt Serva Blue G Xylene Brilliant Cyanine G C I 42655 Coomassie Brilliant

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Coomassie – Wikipedie

Coomassie (nebo tak Brilliant Blue Brilliant Blue G Acid Blue 90 C I 42655 Brilliant Blue G 250) je modr barvivo použvan převžně při elektroforze na polyakrylamidovm gelu v přtomnosti dodecylsranu sodnho (tzn při metodě SDS-PAGE) Gel se v barvivu namč třicet minut a potom se přes třicet minut promv vsledkem je nosič kter umožňuje dokzat

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Coomassie Brilliant Blue : definition of Coomassie

Coomassie Brilliant Blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry Coomassie Brilliant Blue G-250 differs from Coomassie Brilliant Blue R

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How to Choose between G250 or R250 Coomassie Dyes

For these reasons most researchers favor the Coomassie staining technique instead Coomassie Dyes 101: Understanding the Basics Coomassie stains (also known as Coomassie Blue or Coomassie Brilliant Blue) are the most popular anionic protein dyes used in visualizing proteins in SDS-PAGE gels

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An accurate and sensitive Coomassie Brilliant Blue G

We have previously proposed the binding mechanism of Coomassie Brilliant Blue G-250 (CBB) on proteins and developed a novel ultrasensitive method which employs ammonium sulfate and trichloroacetic acid (kosmotropic and protein precipitating agents respectively) These increase the entropy (water disordering) and the hydrophobic interactions

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Homemade Colloidal Coomassie Blue Staining

Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250 Electrophoresis 1988 Jun 9(6):255-62 M Induced Uninduced Purified

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Coomassie Brilliant Blue G 250 › SERVA Electrophoresis

Corresponds to SERVA Blue G ( no 35050) SERVA InfoMail - Get the latest information Close window When subscribing to SERVA InfoMail you will receive information about the latest product release special promotions new jobs at SERVA and more

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G

Coomassie Brilliant Blue G-250 Stain 1L STORAGE CONDITION It is shipped at ambient temperature Store it at room temperature upon arrival and is stable for 1 year when stored and used properly Instructions for Staining the Polyacrylamide Gels: 1 Wash gel 2-3 times 5 minutes each in de-ionized water to remove SDS present in the gel

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G

Coomassie Brilliant Blue G-250 Stain 1L STORAGE CONDITION It is shipped at ambient temperature Store it at room temperature upon arrival and is stable for 1 year when stored and used properly Instructions for Staining the Polyacrylamide Gels: 1 Wash gel 2-3 times 5 minutes each in de-ionized water to remove SDS present in the gel

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Coomassie Brilliant Blue

Coomassie Brilliant Blue R-250/ G-250 are both offered as dry powder form and convenient ready-to-use solutions Destaining solutions are also offered Coomassie Brilliant Blue G-250 Dye Proteomics Grade Cas #: 6104-58-1 Molecular weight: 854 04 Molecular Formula: C47H48N3O7

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THE OF CHEMISTRY Vol 260 No 18 Issue of August 25 pp

capacity for interaction with Coomassie Brilliant Blue G-250 (Coomassie G) in solution Scatchard analysis showed that the number of Coomassie R ligands bound to each protein molecule is approximately proportional to the number of positive charges on the protein about 1 5-3 dye molecules/charge

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Coomassie Blue (R

Coomassie Blue is widely used in visualizing proteins separated by either agarose or acrylamide gel electrophoresis The more popular is Coomassie R250 (Reddish tinted blue) for electrophoresis (more sensitive: can detect as little as 0 1 g of protein) and Coomassie G250 (Greenish tinted blue) for protein assay in solutions (because it is more

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BRILLIANT BLUE G 250 (coomassie brilliant blue G 250

SYNONYMS: C I 42655 Coomassie Brilliant Blue G 250 Acid blue 90 577-584 nm Product Code PB02230 Grade LR CAS No 6104-58-1 Molecuar Formula C47H48N3O7S2Na Molecular weight 854 03 H S Code 32049000 Packings 25gm Application Used to dye wool silk nylon and leather etc Stain for the proteins electrophoresis rapid

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[The Coomassie Brilliant Blue G 250 Method for Protein

[The Coomassie Brilliant Blue G 250 Method for Protein Quantitation (2) The Application of an Automatic Technic (Author's Transl)] Clipboard Search History and several other advanced features are temporarily unavailable Skip to main page content

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Brilliant Blue G (CAS 6104

Coomassie Brilliant Blue G Biological description Potent non-competitive antagonist of rat and human P2X 7 receptors (IC 50 values are 10 and 200 nM respectively) Displays 1000-fold selectivity over P2X 4 IC 50 values for inhibition of the other P2X receptors range from 2 to 30 M

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Coomassie Brilliant Blue G

3/15/2017The Bradford reagent comprised of the Coomassie Brilliant Blue G-250 dye methanol and phosphoric acid has been traditionally used for quantifying proteins Use of this reagent in the Bradford assay relies on the binding of the Coomassie Blue G-250 dye to proteins However the ability of the dye to react with a small group of amino acids (arginine histidine lysine phenylalanine

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Fast and Sensitive Colloidal Coomassie G

Neuhoff V Arold N Taube D Ehrhardt W Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250 Electrophoresis 9 255-262 (1988)

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Recovery of protein by Coomassie Brilliant Blue

The interaction of protein with Coomassie Brilliant Blue G‐250 results in formation of an insoluble protein‐dye complex which can be recovered by centrifugation and redissolved for electrophoretic analysis The precipitated protein can be washed in acetone to

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Coomassie Blue G250 C I 42655

Coomassie Blue dyes are commonly used stains for the detection of proteins bands following gel electrophoresis (SDS-PAGE) Coomassie G-250 offers a faster staining protocol and eliminates the need for destaining the gel Synonyms: Coomassie blue G250 Brilliant blue G250 Acid blue

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Colloidal Coomassie stain

5/19/2015Coomassie Brilliant Blue G-250 (CAS number 6104-58-1) differs from R-250 by inclusion of two methyl groups as shown below in Formula 2 Generic terms for Coomassie Brilliant Blue R-250 (CAS number 6104-59-2) include Xylene Brilliant Cyanine G as well as C I 42655 C I Acid Blue 90 Brilliant indocyanine G and Brillantindocyanin G

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Coomassie Brilliant Blue

Thermo Scientific Pierce Coomassie Brilliant Blue G-250 is one of the most common forms of coomassie dye which is a key component of various colorimetric protein gel stains Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound

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