Agar Facts for Kids

Agar or agar-agar is a jelly-like substance obtained from algae It was discovered in the late 1650s or early 1660s by Mino Tarōzaemon in Japan where it is called kanten Agar is derived from the polysaccharide agarose which forms the supporting structure in the cell walls of certain species of algae and which is released on boiling These algae are known as agarophytes and belong to the

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Growing Bacteria in Petri Dishes

Pour the hot agar mixture back into the dishes (cover them!) as you did before and it should solidify within an hour 6 It's time to collect some bacteria on the end of a cotton swab! The classic test is to roll a clean cotton swab in your mouth and then to lightly draw a squiggle with it on the gelled agar However many people like to test something even more gross like the keys on a

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Resources

If you use the dataset for your research please cite this article as: M Savardi A Ferrari A Signoroni Automatic hemolysis identification on aligned dual-lighting images of cultured blood agar plates Computer Methods and Programs in Biomedicine Vol 156 2018 pp 13-24

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Agar Flakes and Powder

How to Use Agar Powder Stir the agar powder into any cool liquid until it dissolves Heat it in a pan over low heat and allow it to bubble for 45 seconds Then add it to the mixture you wish to thicken In a recipe use use one-third the amount of powder that you would use for flakes (So if the recipe calls for 3 teaspoons agar flakes use 1 teaspoon agar powder ) Use the same amount of agar

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Blood Agar Plates

Blood Agar Plates (BAP) Blood agar is actually a couple of related media all of which are rich formulations containing peptones yeast extracts liver or heart extracts (depending on the medium) and blood The blood is usually sheep's red blood cells (RBC) though horse and other species may be used

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Blood Agar Bacterial Growth Medium

Blood agar (BAP) is a differential growth medium which microbiologists use to distinguish clinically significant bacteria from throat and sputum cultures BAP contains 5% sheep blood Certain bacteria produce exotoxins called hemolysins which act on the red blood cells to lyse or break them down

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Basic microbiology 101: Different types of agar for

Tryptic Soy Agar (TSA) - A general type of agar It cultivates non-fastidious bacteria such as Staphylococcus Aureus Eli Pseudomonas Aeriginosa Chocolate Agar - This is an enriched agar It cultivates fastidious organisms such as Neisseria or Haemophilus Sp The agar is been named like that because of the color It does not contain chocolate

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Tributyrin Agar Base

3 0 agar-agar 12 0 Also to be added: Tributyrin 10 0 ml Preparation Suspend 20 g/litre add 10 ml neutral tributyrin/litre mix uniformly and autoclave (15 min at 121 C) While shaking frequently (emulsification of the tributyrin) cool to at least 50C (stabilization of the emulsion) and pour plates Allow the plates to solidify rapidly

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Bacteria Growing Experiments in Petri Plates

Blood agar or tryptic soy agar with 5% sheep's blood is an excellent medium for supplying bacteria with nutrients and an environment in which we can see them grow Sterile powdered agar with nutrients can be mixed with water heated and then poured into empty petri plates or ready-to-use

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2: Aseptic Transfers

Use an inoculating needle for agar deeps and an inoculating loop for agar plates and broths ALWAYS use the proper aseptic technique when transferring cultures from one medium to another Be sure to put any extra media tubes BACK into the racks if unused: Replace agar plates back into bags if not used

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How to Make Skim Milk Agar Plates

Skim milk agar plates are used to provide a nutritious medium for growing micro-organisms Once prepared the agar can be plated with a population of micro-organisms to test for the micro-organism's ability to digest casein protein Casein is a large insoluble protein found in skim milk As it

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EZ BioResearch Bacteria Science Kit (10

We use Luria Broth (LB) based medium in our agar plates LB medium is a nutrient rich medium which allows fast and proliferative growth of bacteria LB is the most commonly used medium in the research laboratories all over the world It is safe for children/students to use due to its nonselectivity - Not all bacteria growth media are safe for children/students to use Tryptic Soy (TS) medium

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Antibiotic Sensitivity Bacteria Screening

Make Agar plates the day before the experiment Agar plates can be made up to aweek in advance stored in an airtight container at 4qC 1 Add the entire pack of LB Agar to an autoclavable container and add 150ml distilled water Autoclave for 15min at 121qC 2 Once the LB Agar has cooled to handle (about 45qC) pour a ~0 5cm / layer of

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Storage Use and Shipping of Surface Contact (RODAC

Do not use expired plates Preparation Prepare a test diagram for the areas that are to be tested and label plates with the corresponding location identification Ensure that the label cannot be readily wiped off or removed RODAC plates are prepared so that the agar surface is convex for sampling flat surfaces

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Oxoid

Dry the surface of agar plates for a minimal period of time prior to use With a glass spatula spread 0 1ml aliquots of food dilutions made up in Buffered Peptone Water on the agar surface until it is dry Up to 0 5ml may be used on larger dishes Incubate the inverted dishes at 35C

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How To Make Agar and Kanten • Just One Cookbook

If you are using agar/kanten stick you will need to rinse it carefully and break it into pieces to soak them in water for 30 minutes This will help dissolve agar/kanten faster Squeeze the water out from the pieces and they are ready to use Watch How to Make Agar のり

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How To Streak a Bacterial Culture

When sterilizing the inoculating loop make sure that the entire loop turns orange before using on the agar plates When streaking the agar with the loop be sure to keep the loop horizontal and only streak the surface of the agar If using sterile toothpicks use a new toothpick when performing each new streak Throw all used toothpicks away Safety: When growing bacterial colonies you will

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Making a spread or 'lawn' plate

4 Use agar plates with a well-dried surface so that the inoculum dries quickly Dry the surface of agar plates by incubating for several hours (perhaps overnight) or put them in a hot air oven (at 55-60 C) for 30-60 minutes with the two halves separated and the inner surfaces directed downwards

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